Shelf stability study of granules from Pleurotus ostreatus mushroom
Keywords:
pharmaceutical stability; granules; quality control; Pleurotus ostreatus; mushroom.
Abstract
The most straightforward solid dosage form comprises granules prepared from the drug and other stable components in a size that allows its precise handling and bulk dispensing. The increasing prevalence of a multitude of ailments has prompted the utilization of novel therapeutic agents, such as Pleurotus ostreatus mushroom. The objective of the present study was to evaluate the physical, chemical, and microbiological stability of a Pleurotus ostreatus granulate over a 12-month period. An experimental study was conducted to assess the quality through physical, chemical and microbiological parameters over one-year period for three batches of the granulate. The results demonstrated the presence of a heterogeneous granulate with a distinctive aroma and flavor profile, typical of dried mushrooms, and a brown coloration. The residual moisture exhibited a slight increase (2, 85–4,70 %), while a decrease of 5 % in phenolic concentration was observed. Additionally, the microbiological analysis did not indicate the presence of pathogenic microorganisms. The pharmaceutical stability of all three evaluated batches was maintained throughout the study period
References
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ISSN 0719-4250
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Mushrooms [en línea]. 2014, 16 (3). 239-245. DOI:
10.1615/IntJMedMushr.v16.i3.40
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Walter A. Estudio preliminar de la composición nutricional del hongo Pleurotus
ostreatus cultivado en pulpa de café. Revista de la Sociedad Química del Perú
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0-12-820007-0.00019-2
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granulation of pharmaceuticals and excipients: Principle, analysis and typical
applications. Powder Technol [en línea]. 2022, 401 (117329). DOI:
10.1016/j.powtec.2022.117329
19. ARSHAD MS, Zafar S, Yousef B, Alyassin Y, Ali R, Al Asiri A, et al. A review
of emerging technologies enabling improved solid oral dosage form
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fruiting bodies extracts from the oyster mushroom Pleurotus ostreatus. Journal
of the International Society of Antioxidants in Nutrition and Health [en línea].
2015 1(1). DOI: 10.18143/JISANH_v1i1
23. DE, Arnab, et al. Pharmacopoeias, national formulary and extra
pharmacopoeia. En: Amit Kumar Nayak, Kalyan Kumar Sen, Dosage Forms,
Formulation Developments and Regulations: Academic Press, 2024, pp. 83-98.
ISBN 9780323918176
24. GARCÍA-GONZÁLEZ, M. C., Crespo-Zafra, L. M., Macías-González, A. A.,
& Varela-De Moya, H. S. Fortificación de las harinas de cereales con harinas de
Pleurotus ostreatus. Revista científica multidisciplinaria arbitrada yachasun [en
línea]. 2023, 7(12), 188–205. ISSN: 2697-3456.
25. JUBERÍAS SÁNCHEZ, A., et al. Estudio de prototipos de formulaciones
farmacéuticas de soluciones hidratantes para situaciones de estrés hídrico.
Sanidad Militar [en línea]. 2019, 75(2). 73-79. DOI: 10.4321/s1887-
85712019000100003
26. AHIRWAR K, Shukla R. Preformulation studies: a versatile tool in
formulation design. In: Shukla R, Kuznetsov A, Ali A, editors. Drug Formulation
Design. London: IntechOpen; 2023. DOI: 10.5772/intechopen.105307
27. MORRIS, H J. et al. Mycelia from Pleurotus sp. (oyster mushroom): a new
wave of antimicrobials, anticancer and antioxidant bio-ingredients. International
Journal of Phytocosmetics and Natural Ingredients [en línea]. 2017, 4(1). 3.
DOI: 10.15171/ijpni.2017.03
28. MORRIS, H J., et al. Mushroom science in Cuba: towards new opportunities
for developing functional foods/nutraceuticals. En: Proceedings of the 8th
International Conference on Mushroom Biology and Mushroom Products
(ICMBMP8), November 19-22, New Delhi, India, 2014, II, pp. 422-432.
29. MUSZYNSKA, Bozena; SULKOWSKA-ZIAJA, Katarzyna; EKIERT, Halina.
Phenolic acids in selected edible basidiomycota species: Armillaria mellea,
Boletus badius, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus and Pleurotus ostreatus. Acta Scientiarum Polonorum. Hortorum Cultus [en línea].
2013, 12 (4). 107-116. ISSN 1644-0692
30. FINIMUNDY, Tiane C., et al. Aqueous extracts of Lentinula edodes and
Pleurotus sajor-caju exhibit high antioxidant capability and promising in vitro
antitumor activity. Nutrition Research [en línea]. 2013, 33(1). 76-84. DOI:
10.1016/j.nutres.2012.11.005
31.WAKTOLA, Girma; TEMESGEN, Tasisa. Pharmacological activities of
Oyster mushroom (Pleurotus ostreatus). Novel Research in Microbiology
Journal [en línea]. 2020, 4 (2). 688-695. DOI: 10.21608/nrmj.2020.84017
32. MKHIZE, Senzosenkosi Surprise, et al. The effect of supplementing
mushroom growing substrates on the bioactive compounds, antimicrobial
activity, and antioxidant activity of Pleurotus ostreatus. Biochemistry Research
International [en línea]. 2022, 2022 (1). 9436614. DOI: 10.1155/2022/9436614
33. MISHRA, Vartika, et al. Elemental analysis, phytochemical screening and
evaluation of antioxidant, antibacterial and anticancer activity of Pleurotus
ostreatus through in vitro and in silico approaches. Metabolites [en línea]. 2022,
12 (9). 821. DOI: 10.3390/metabo12090821
dosage form development. New York: Informa Healthcare CRC Press, 2008.
ISBN 978-0-8247-5809-7
2. GIBSON, M. Pharmaceutical Preformulation and Formulation. Second
Edition. London: Informa Healthcare CRC Press, 2009.
ISBN 978-1-4200-7317-1
3. HICKEY, A. GANDERTON, D. Pharmaceutical Process Engineering. Second
Edition. London: Informa Healthcare CRC Press, 2010.
ISBN 978-1-4200-8475-7
4. PETROVIĆ, Jovana, et al. Nutritional value, chemical composition,
antioxidant activity and enrichment of cream cheese with chestnut mushroom
Agrocybe aegerita (Brig.) Sing. Journal of food science and technology [en
línea]. 2015, 52. 6711-6718. DOI: 10.1007/s13197-015-1783-6
5. MORRIS, H J, et al. The use of mushrooms in the development of functional
foods, drugs, and nutraceuticals. In: Ferreira I, Barros L, Morales P. (Eds.): Wild
plants, mushrooms and nuts: Functional Food properties and applications. First
Edition. John Wiley & Sons, Ltd., Chichester, UK; Hoboken, NJ, 2017, pp. 123-
157. ISBN 9781118944653
6. BELTRÁN-DELGADO, Yaixa., et al. Procedimientos para la producción de
setas del género Pleurotus con potencial aplicación farmacológica. Revista
Cubana de Química [en línea]. 2020, 32 (2). 245-261. ISSN 2224-5421
7. ARANGO, Carolina Suárez; NIETO, Ivonne Jeannette. Cultivo biotecnológico
de macrohongos comestibles: una alternativa en la obtención de nutracéuticos.
Revista Iberoamericana de Micología [en línea]. 2013, 30 (1). 1-8.
DOI: 10.1016/j.riam.2012.03.011
8. BERMÚDEZ SAVÓN, Rosa Catalina, et al. Utilización de cáscara (fibra) de
coco para el cultivo de setas comestibles-medicinales de interés comercial.
Tecnología Química [en línea]. 2020, 40 (2). 260-268. ISSN 2224-6185
9. PISKA, Kamil; SUŁKOWSKA-ZIAJA, Katarzyna; MUSZYŃSKA, Bożena.
Edible mushroom Pleurotus ostreatus (oyster mushroom): its dietary
significance and biological activity. Acta Scientiarum Polonorum. Hortorum
Cultus [en línea]. 2017, 16 (1). 151-161. ISSN 1644-0692
10. LLAURADO, Gabriel, et al. Restoration of liver function in malnourished
mice orally administered with Pleurotus ostreatus fruiting bodies extract. Journal
of Pharmacy & Pharmacognosy Research [en línea]. 2020, 8 (1). 32-42.
ISSN 0719-4250
11. BELTRÁN-DELGADO, Yaixa, et al. Acoplamiento molecular de compuestos
fenólicos de Pleurotus ostreatus con proteínas del balance redox. Revista
Cubana de Química [en línea]. 2019, 31 (3). 336-353. ISSN 2224-5421
12. MORRIS, H J., et al. In vitro anti-proliferative effects on NB4 human
leukemia cells and physicochemical screening of Pleurotus sp. (higher
Basidiomycetes) mycelia from Cuba. International Journal of Medicinal
Mushrooms [en línea]. 2014, 16 (3). 239-245. DOI:
10.1615/IntJMedMushr.v16.i3.40
13. NIETO-JUÁREZ, Jessica I.; CUZCANO-RUIZ, Ángel D.; REYES-LÓPEZ,
Walter A. Estudio preliminar de la composición nutricional del hongo Pleurotus
ostreatus cultivado en pulpa de café. Revista de la Sociedad Química del Perú
[en línea]. 2019, 85 (4). 422-431. ISSN 1810-634X
14. LARA, Jhenifer Daniela., et al. Actividad antibacteriana de Pleurotus
ostreatus. Compendio Investigativo de Academia Journals Celaya [en línea].
2017, p. 990-995. ISBN: 978-1-939982-32-2
15. ARIAS-RAMOS, Daily, et al. Technological, biochemical and microbiological
evaluation of dehydrated Pleurotus ostreatus powder for nutraceutical
applications. Current Nutrition & Food Science [en línea]. 2022, 18 (5). 507-514.
DOI: 10.2174/1573401318666220211101143
16. LOZANO ESTEVAN, María del Carmen; CÓRDOBA DÍAZ, Damián;
CÓRDOBA, Manuel. Manual de tecnología farmacéutica. Manual de tecnología
farmacéutica. España: Elsevier, 2012, pp. 51-79. ISBN 978-84-8086-600-2
17. AWAD A, Trenfield SJ, Basit AW. Solid oral dosage forms. In: Adejare A,
editor. Remington -The Science and Practice of Pharmacy. 23rd ed.
Cambridge: Academic Press (Elsevier); 2021. pp. 333-58. DOI: 10.1016/B978-
0-12-820007-0.00019-2
18. WANG B, Sun X, Xiang J, Guo X, Cheng Z, Liu W, et al. A critical review on
granulation of pharmaceuticals and excipients: Principle, analysis and typical
applications. Powder Technol [en línea]. 2022, 401 (117329). DOI:
10.1016/j.powtec.2022.117329
19. ARSHAD MS, Zafar S, Yousef B, Alyassin Y, Ali R, Al Asiri A, et al. A review
of emerging technologies enabling improved solid oral dosage form
manufacturing and processing. Advanced drug delivery reviews [en línea].
2021, 178. 113840. DOI: 10.1016/j.addr.2021.113840
20. DEBESA GARCÍA, Francisco; FERNÁNDEZ ARGÜELLES, Rogelio;
PÉREZ PEÑA, Julián. La caducidad de los medicamentos: justificación de una
duda. Revista Cubana de Farmacia [en línea]. 2004, 8(3). 1-1. ISSN 1561-2988
21. ROWE, Raymond C.; SHESKEY, Paul; QUINN, Marian. Handbook of
pharmaceutical excipients. Argentina: Libros Digitales-Pharmaceutical Press,
2009. ISBN 978 1 58212 135 2
22. BELTRÁN, Yaixa, et al. Phenolic content and invitro antioxidant activities of
fruiting bodies extracts from the oyster mushroom Pleurotus ostreatus. Journal
of the International Society of Antioxidants in Nutrition and Health [en línea].
2015 1(1). DOI: 10.18143/JISANH_v1i1
23. DE, Arnab, et al. Pharmacopoeias, national formulary and extra
pharmacopoeia. En: Amit Kumar Nayak, Kalyan Kumar Sen, Dosage Forms,
Formulation Developments and Regulations: Academic Press, 2024, pp. 83-98.
ISBN 9780323918176
24. GARCÍA-GONZÁLEZ, M. C., Crespo-Zafra, L. M., Macías-González, A. A.,
& Varela-De Moya, H. S. Fortificación de las harinas de cereales con harinas de
Pleurotus ostreatus. Revista científica multidisciplinaria arbitrada yachasun [en
línea]. 2023, 7(12), 188–205. ISSN: 2697-3456.
25. JUBERÍAS SÁNCHEZ, A., et al. Estudio de prototipos de formulaciones
farmacéuticas de soluciones hidratantes para situaciones de estrés hídrico.
Sanidad Militar [en línea]. 2019, 75(2). 73-79. DOI: 10.4321/s1887-
85712019000100003
26. AHIRWAR K, Shukla R. Preformulation studies: a versatile tool in
formulation design. In: Shukla R, Kuznetsov A, Ali A, editors. Drug Formulation
Design. London: IntechOpen; 2023. DOI: 10.5772/intechopen.105307
27. MORRIS, H J. et al. Mycelia from Pleurotus sp. (oyster mushroom): a new
wave of antimicrobials, anticancer and antioxidant bio-ingredients. International
Journal of Phytocosmetics and Natural Ingredients [en línea]. 2017, 4(1). 3.
DOI: 10.15171/ijpni.2017.03
28. MORRIS, H J., et al. Mushroom science in Cuba: towards new opportunities
for developing functional foods/nutraceuticals. En: Proceedings of the 8th
International Conference on Mushroom Biology and Mushroom Products
(ICMBMP8), November 19-22, New Delhi, India, 2014, II, pp. 422-432.
29. MUSZYNSKA, Bozena; SULKOWSKA-ZIAJA, Katarzyna; EKIERT, Halina.
Phenolic acids in selected edible basidiomycota species: Armillaria mellea,
Boletus badius, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus and Pleurotus ostreatus. Acta Scientiarum Polonorum. Hortorum Cultus [en línea].
2013, 12 (4). 107-116. ISSN 1644-0692
30. FINIMUNDY, Tiane C., et al. Aqueous extracts of Lentinula edodes and
Pleurotus sajor-caju exhibit high antioxidant capability and promising in vitro
antitumor activity. Nutrition Research [en línea]. 2013, 33(1). 76-84. DOI:
10.1016/j.nutres.2012.11.005
31.WAKTOLA, Girma; TEMESGEN, Tasisa. Pharmacological activities of
Oyster mushroom (Pleurotus ostreatus). Novel Research in Microbiology
Journal [en línea]. 2020, 4 (2). 688-695. DOI: 10.21608/nrmj.2020.84017
32. MKHIZE, Senzosenkosi Surprise, et al. The effect of supplementing
mushroom growing substrates on the bioactive compounds, antimicrobial
activity, and antioxidant activity of Pleurotus ostreatus. Biochemistry Research
International [en línea]. 2022, 2022 (1). 9436614. DOI: 10.1155/2022/9436614
33. MISHRA, Vartika, et al. Elemental analysis, phytochemical screening and
evaluation of antioxidant, antibacterial and anticancer activity of Pleurotus
ostreatus through in vitro and in silico approaches. Metabolites [en línea]. 2022,
12 (9). 821. DOI: 10.3390/metabo12090821
Published
2025-05-21
How to Cite
Arias-Ramos, D. A.-R., Chil-Núñez, I. C.-N., & Morris-Quevedo, H. J. M.-Q. (2025). Shelf stability study of granules from Pleurotus ostreatus mushroom. Chemical Technology, 45, 159-175. Retrieved from https://tecnologiaquimica.uo.edu.cu/index.php/tq/article/view/5456
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