BIOTECHNOLOGICAL POTENTIAL OF COFFEE PULP LIGNINOLITIC ENZYMES TO PRODUCE FOR FES

Abstract

Biotechnological potential of coffee pulp as substrate for extracellular ligninolytic enzyme production by solid state fermentation (SSF) using five of Pleurotus ostreatus strains and one Pleurotus sajor-caju strain, after fructification state without mushrooms productions affectation. Maximum laccase production was detected and Pleurotus ostreatus CCEBI 3023 showed a higher laccase activity (0,139 U g-1). Influence of mixture (1:1) coffee pulp whit cedar chip, coconut and cocoa shell in the laccase production was evaluated. The coffee pulp induced extracellular laccase enzyme production because whit this substrate the maximum laccase activity was obtained (1,745 U g-1) at 60 days of fermentation whit Pleurotus ostreatus CCEBI 3023 strain. The results of this investigation were compared whit the study realized in vegetative state of Pleurotus ostreatus CCEBI 3023 using coffee pulp and whit laccase production of Pleurotus sp. by submerged fermentation.